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KMID : 0545120150250071108
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 7 p.1108 ~ p.1113
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Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration
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Kim Hyun-Joong
Kim Yong-Hyun
Shin Ji-Hyun
Shashi Kant Bhatia
Ganesan Sathiyanarayanan
Seo Hyung-Min
Choi Kwon-Young
Yang Yung-Hun
Park Kyung-Moon
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Abstract
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Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using a recombinant Escherichia coli strain overexpressing the E. coli MG1655 cadA gene, and various parameters were investigated for the whole-cell bioconversion of lysine to cadaverine. A high concentration of lysine resulted in the synthesis of pyridoxal-5’-phosphate (PLP) and it was found to be a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, consumption of 91% lysine and conversion of about 80% lysine to cadaverine were successfully achieved.
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KEYWORD
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Biotransformation, whole-cell biocatalyst, lysine decarboxylase, cadaverine, high-concentration reaction, Escherichia coli
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